Article
High-throughput RNAi screening in cultured cells: a user's guide.
Nature reviews. Genetics - 1 May 2006
Echeverri Christophe J, Perrimon Norbert
Abstract excerpt
RNA interference has re-energized the field of functional genomics by enabling genome-scale loss-of-function screens in cultured cells. Looking back on the lessons that have been learned from the first wave of technology developments and applications in this exciting field, we provide both a user's guide for newcomers to the field and a detailed examination of some more complex issues, particularly concerning...
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