Article

Multiplexed pooled library screening with Cpf1

2018-11-19

Abstract excerpt

RNA interference and CRISPR/Cas9-based pooled library screens have revolutionized the field of functional genomics. However, currently available pooled library screens face a trade-off between library effectiveness and library complexity. We developed a multiplexed, high-throughput screening strategy based on an optimized AsCpf1 nuclease that minimizes library size without sacrificing gene targeting efficiency. Ou...

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Identifiers and source

Literature Corpus work: a6326161-d467-5be7-a287-9e77e9b8d160
DOI: 10.1101/473959

Open publication

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