Article
An aspartyl cathepsin, CTH3, is essential for proprotein processing during secretory granule maturation in Tetrahymena thermophila.
Molecular biology of the cell - 15 Aug 2014
Kumar Santosh, Briguglio Joseph S, Turkewitz Aaron P
Abstract excerpt
In Tetrahymena thermophila, peptides secreted via dense-core granules, called mucocysts, are generated by proprotein processing. We used expression profiling to identify candidate processing enzymes, which localized as cyan fluorescent protein fusions to mucocysts. Of note, the aspartyl cathepsin Cth3p plays a key role in mucocyst-based secretion, since knockdown of this gene blocked proteolytic maturation of the...
Topics
- Carboxypeptidases
- Cathepsins
- Exocytosis
- Mutation
- Phylogeny
- Secretory Vesicles
- Tetrahymena thermophila