Article
Analysis of a mutant exhibiting conditional sorting to dense core secretory granules in Tetrahymena thermophila.
Genetics - 1 Dec 2001
Bowman G R, Turkewitz A P
Abstract excerpt
The formation of dense core granules (DCGs) requires both the sorting of granule contents from other secretory proteins and a postsorting maturation process. The Tetrahymena thermophila strain SB281 fails to synthesize DCGs, and previous analysis suggested that the defect lay at or near the sorting step. Because this strain represents one of the very few mutants in this pathway, we have undertaken a more complete...
Topics
- Animals
- Blotting, Western
- Cell Division
- Centrifugation, Density Gradient
- Cycloheximide
- Cytoplasm
- Epistasis, Genetic
- Fluorescent Antibody Technique, Indirect
- Genetic Techniques
- Microscopy, Fluorescence
- Models, Genetic