Article
Maturation of dense core granules in wild type and mutant Tetrahymena thermophila.
The EMBO journal - 1 Aug 1991
Turkewitz A P, Madeddu L, Kelly R B
Abstract excerpt
Tetrahymena thermophila, a ciliated protozoan, has a well-developed pathway of regulated secretion from dense core granules called mucocysts. Since exocytosis-defective mutants are available, steps in the biogenesis of dense core granules and their fusion with the plasma membrane may be resolved genetically. To describe the steps in biochemical terms, we have generated antisera against mucocyst content proteins....
Topics
- Animals
- Blotting, Western
- Calcium-Binding Proteins
- Cytoplasmic Granules
- Electrophoresis, Polyacrylamide Gel
- Exocytosis
- Microscopy, Electron
- Mutation
- Precipitin Tests
- Protozoan Proteins
- Tetrahymena