Article
A Spinach molecular beacon triggered by strand displacement.
RNA (New York, N.Y.) - 1 Aug 2014
Bhadra Sanchita, Ellington Andrew D
Abstract excerpt
We have re-engineered the fluorescent RNA aptamer Spinach to be activated in a sequence-dependent manner. The original Spinach aptamer was extended at its 5'- and 3'-ends to create Spinach.ST, which is predicted to fold into an inactive conformation and thus prevent association with the small molecule fluorophore DFHBI. Hybridization of a specific trigger oligonucleotide to a designed toehold leads to...
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