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Systematic Mutation and Unnatural Base Pair Incorporation Improves Riboswitch-Based Biosensor Response Time.

ACS sensors - 22 Dec 2023

Manna Sudeshna, Kimoto Michiko, Truong Johnny, Bommisetti Praneeth, Peitz Ava, Hirao Ichiro, Hammond Ming C

Abstract excerpt

Engineered RNAs have applications in diverse fields from biomedical to environmental. In many cases, the folding of the RNA is critical to its function. Here we describe a strategy to improve the response time of a riboswitch-based fluorescent biosensor. Systematic mutagenesis was performed to either make transpose or transition base pair mutants or introduce orthogonal base pairs. Both natural and unnatural base...

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PMID: 37878677
DOI: 10.1021/acssensors.3c01266
PMCID: PMC10749561

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