Isolation and characterization of fluorescence-enhancing RNA tags.

doi:10.1016/j.bmc.2010.08.006

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Article

Isolation and characterization of fluorescence-enhancing RNA tags.

Bioorganic & medicinal chemistry - 1 Feb 2011

Wiesmayr Anna, Jäschke Andres

Abstract excerpt

Methods for the visualization of RNAs are urgently needed for studying a wide variety of cellular processes. Here we report on-bead screening of RNA libraries and its application to the isolation of specific fluorescence-enhancing RNA sequences. A one-bead-one-compound combinatorial RNA library with over one million different sequences was synthesized using the split-and-mix method. Solid-phase synthesis of 30...

Topics

Base Sequence
Fluorescence
Fluorescent Dyes
Mutation
Polystyrenes
RNA
Sequence Analysis, RNA

Source

Open in PubMed

Identifiers

PMID: 20813536
DOI: 10.1016/j.bmc.2010.08.006

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