Article
A fluorescent molecular switch for room temperature operation based on oligonucleotide hybridization without labeling of probes or targets.
Analytica chimica acta - 31 Oct 2012
Massey Melissa, Krull Ulrich J
Abstract excerpt
A molecular switch was prepared by self-assembly. Neutravidin served as a template that allowed for a biotinylated probe oligonucleotide to be placed adjacent to a biotinylated long-chain linker that was terminated with thiazole orange (TO). Hybridization of probe oligonucleotide with target to form double-stranded DNA resulted in intercalation of the adjacent TO probe. This was a reversible process that could be...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.