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Article

Single strain isolation method for cell culture-adapted hepatitis C virus by end-point dilution and infection.

PloS one - 1 Jan 2014

Sugiyama Nao, Murayama Asako, Suzuki Ryosuke, Watanabe Noriyuki, Shiina Masaaki, Liang T Jake, Wakita Takaji, Kato Takanobu

Abstract excerpt

The hepatitis C virus (HCV) culture system has enabled us to clarify the HCV life cycle and essential host factors for propagation. However, the virus production level of wild-type JFH-1 (JFH-1/wt) is limited, and this leads to difficulties in performing experiments that require higher viral concentrations. As the cell culture-adapted JFH-1 has been reported to have robust virus production, some mutations in the...

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Identifiers

PMID: 24848954
DOI: 10.1371/journal.pone.0098168
PMCID: PMC4029950

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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