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Article

Advantages of a single-cycle production assay to study cell culture-adaptive mutations of hepatitis C virus.

Proceedings of the National Academy of Sciences of the United States of America - 18 Mar 2008

Russell Rodney S, Meunier Jean-Christophe, Takikawa Shingo, Faulk Kristina, Engle Ronald E, Bukh Jens, Purcell Robert H, Emerson Suzanne U

Abstract excerpt

The JFH1 strain of hepatitis C virus (HCV) is unique among HCV isolates, in that the wild-type virus can traverse the entire replication cycle in cultured cells. However, without adaptive mutations, only low levels of infectious virus are produced. In the present study, the effects of five mutations that were selected during serial passage in Huh-7.5 cells were studied. Recombinant genomes containing all five...

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Identifiers

PMID: 18334634
DOI: 10.1073/pnas.0800422105
PMCID: PMC2393785

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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