Article
Stability curves of laboratory evolved thermostable mutants of a Bacillus subtilis lipase.
Biochimica et biophysica acta - 1 Sept 2010
Kamal Md Zahid, Ahmad Shoeb, Yedavalli Poornima, Rao Nalam Madhusudhana
Abstract excerpt
Shape of the protein stability curves changes to achieve higher melting temperature. Broadly, these changes have been classified as upward shift (increased G(s)), rightward shift (increase in T(s)) and flattening of the stability curves (decrease in C(p)). Comparative studies on homologous mesophilic-thermophilic protein pairs highlighted the differential contribution of these three strategies amongst proteins....
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