Machine learning-guided multi-site combinatorial mutagenesis enhances the thermostability of pectin lyase.

Abstract excerpt

Enhancing the thermostability of enzymes is crucial for industrial applications. Methods such as directed evolution are often limited by the huge sequence space and combinatorial explosion, making it difficult to obtain optimal mutants. In recent years, machine learning (ML)-guided protein engineering has become an attractive tool because of its ability to comprehensively explore the sequence space of enzymes and...

Topics

• Polysaccharide-Lyases
• Enzyme Stability
• Machine Learning
• Protein Engineering
• Molecular Dynamics Simulation
• Mutagenesis
• Temperature
• Bacillus licheniformis
• Mutagenesis, Site-Directed
• Mutation