Article
Exploring nonnatural evolutionary pathways by saturation mutagenesis: rapid improvement of protein function.
Journal of molecular evolution - 1 Dec 1999
Miyazaki K, Arnold F H
Abstract excerpt
Random point mutagenesis does not access a large fraction of protein sequence space corresponding to primarily nonconservative amino acid substitutions. The cost of this limitation during directed evolution is unknown. Random point mutagenesis over the entire gene encoding the psychrophilic protease subtilisin S41 identified a pair of residues (Lys211 and Arg212) where mutations provided significant increases in...
Topics
- Amino Acid Substitution
- Bacillus subtilis
- DNA Mutational Analysis
- Enzyme Stability
- Evolution, Molecular
- Genetic Variation
- Mutagenesis
- Mutation
- Polymerase Chain Reaction
- Selection, Genetic
- Structure-Activity Relationship