Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

TSpred: a web server for the rational design of temperature-sensitive mutants.

Nucleic acids research - 1 Jul 2014

Tan Kuan Pern, Khare Shruti, Varadarajan Raghavan, Madhusudhan Mallur Srivatsan

Abstract excerpt

Temperature sensitive (Ts) mutants of proteins provide experimentalists with a powerful and reversible way of conditionally expressing genes. The technique has been widely used in determining the role of gene and gene products in several cellular processes. Traditionally, Ts mutants are generated by random mutagenesis and then selected though laborious large-scale screening. Our web server, TSpred...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Bacterial Proteins
Share to Internet
Share to Muramidase

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Mutation

Share to Proteins

Share to Software

Share to Temperature

Source

Open in PubMed Open full text

Identifiers

PMID: 24782523
DOI: 10.1093/nar/gku319
PMCID: PMC4086094

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

TSpred: a web server for the rational design of temperature-sensitive mutants.PMID 24782523Read ↗

FireProt: web server for automated design of thermostable proteins.Semantic proximity Read ↗

A procedure for the prediction of temperature-sensitive mutants of a globular protein based solely on the amino acid sequence.Semantic proximity Read ↗

Rational design of temperature-sensitive alleles using computational structure prediction.Semantic proximity Read ↗

Selection of mutations for increased protein stability.Semantic proximity Read ↗

Design of temperature-sensitive mutants solely from amino acid sequence.Semantic proximity Read ↗

FireProt 2.0: web-based platform for the fully automated design of thermostable proteins.Semantic proximity Read ↗

Isolation of Temperature-sensitive p53 Mutations from a Comprehensive Missense Mutation LibrarySemantic proximity Read ↗

Structural correlates of the temperature sensitive phenotype derived from saturation mutagenesis studies of CcdB.Semantic proximity Read ↗

Experimental Protocols for Generating Focused Mutant Libraries and Screening for Thermostable Proteins.Semantic proximity Read ↗

Site-saturation mutagenesis: a powerful tool for structure-based design of combinatorial mutation libraries.Semantic proximity Read ↗

Design and utility of temperature-sensitive Gal4 mutants for conditional gene expression in Drosophila.Semantic proximity Read ↗

ProTherm, version 2.0: thermodynamic database for proteins and mutants.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.