Article
Rational design of temperature-sensitive alleles using computational structure prediction.
PloS one - 1 Jan 2011
Poultney Christopher S, Butterfoss Glenn L, Gutwein Michelle R, Drew Kevin, Gresham David, Gunsalus Kristin C, Shasha Dennis E, Bonneau Richard
Abstract excerpt
Temperature-sensitive (ts) mutations are mutations that exhibit a mutant phenotype at high or low temperatures and a wild-type phenotype at normal temperature. Temperature-sensitive mutants are valuable tools for geneticists, particularly in the study of essential genes. However, finding ts mutations typically relies on generating and screening many thousands of mutations, which is an expensive and...
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