Article
A procedure for the prediction of temperature-sensitive mutants of a globular protein based solely on the amino acid sequence.
Proceedings of the National Academy of Sciences of the United States of America - 26 Nov 1996
Varadarajan R, Nagarajaram H A, Ramakrishnan C
Abstract excerpt
Temperature-sensitive (Ts) mutants of a protein are an extremely powerful tool for studying protein function in vivo and in cell culture. We have devised a method to predict those residues in a protein sequence that, when appropriately mutated, are most likely to give rise to a Ts phenotype. Since substitutions of buried hydrophobic residues often result in significant destabilization of the protein, our method...
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