Article
Limitation of high-resolution melting curve analysis for genotyping simple sequence repeats in sheep.
Genetics and molecular research : GMR - 8 Apr 2014
Yang M, Yue Y J, Guo T T, Han J L, Liu J B, Guo J, Sun X P, Feng R L, Wu Y Y, Wang C F, Wang L P, Yang B H
Abstract excerpt
Variation in microsatellite or simple sequence repeat (SSR) loci has, until recently, relied heavily on the use of gel-based methods that can be both time consuming and difficult to genotype. Non gel-based systems are therefore important to increase simplicity and improve turn-around time without compromising assay sensitivity and accuracy. In this report, we assessed the latest of the non-gel-based methods,...
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