Article
Application of high-resolution melting for genotyping bovine mitochondrial DNA.
Biotechnology progress - 1 Jan 2000
Zhou Zai-Wei, Yan Jing-Bin, Li Hua, Ren Zhao-Rui
Abstract excerpt
Recent studies have demonstrated that mitochondrial DNA (mtDNA) haplotype has a significant impact on the efficiency of bovine somatic cell nuclear transfer. Conventional methods for detecting mtDNA variations and haplotypes, such as restriction fragment length polymorphism (RFLP), temporal temperature gradient gel electrophoresis, dHPLC and sequencing, are labor intensive or expensive and have low sensitivity....
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