Article
Improvements in allelic discrimination of microsatellite markers using denaturing polyacrylamide gel electrophoresis.
Mammalian genome : official journal of the International Mammalian Genome Society - 1 Aug 2000
Symons R C, Marshall V M, Foote S J
Abstract excerpt
Poor resolution, retarded progress of DNA through gels, and variable sizing of DNA fragments between and within gels hinder accurate genotyping of some simple sequence length polymorphism (SSLP) markers with the Perkin Elmer Applied Biosystems 377 Sequenator. These problems are similar to renaturation related problems observed in DNA sequencing gels. PCR products especially susceptible to these problems are shown...
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