Article
High-resolution melt analysis of the minisatellite D1S80: a potential forensic screening tool.
Electrophoresis - 1 Nov 2014
Pomeroy Robert S, Balamurugan Kuppareddi, Wong Helena, Duncan George
Abstract excerpt
High-resolution melt (HRM) analysis of the VNTR region of the human D1S80 locus, a 16-bp repeat minisatellite from approximately 400 to over 700 bp in length, was investigated. A Qiagen Rotor-Gene Q using the Type-it PCR HRM kit was used to acquire HRM curves for 14 single, and 16 biallelic, dsDNA samples. The HRM analysis was applicable over a range of DNA concentrations; however the characteristics of the melt...
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