Article
High resolution melting analysis is a more sensitive and effective alternative to gel-based platforms in analysis of SSR--an example in citrus.
PloS one - 1 Jan 2012
Distefano Gaetano, Caruso Marco, La Malfa Stefano, Gentile Alessandra, Wu Shu-Biao
Abstract excerpt
High resolution melting curve analysis (HRM) has been used as an efficient, accurate and cost-effective tool to detect single nucleotide polymorphisms (SNPs) or insertions or deletions (INDELs). However, its efficiency, accuracy and applicability to discriminate microsatellite polymorphism have not been extensively assessed. The traditional protocols used for SSR genotyping include PCR amplification of the DNA...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.