Article
[Comparative analysis of real-time quantitative PCR-Sanger sequencing method and TaqMan probe method for detection of KRAS/BRAF mutation in colorectal carcinomas].
Zhonghua bing li xue za zhi = Chinese journal of pathology - 1 Feb 2014
Zhang Xun, Wang Yuehua, Gao Ning, Wang Jinfen
Abstract excerpt
OBJECTIVE: To compare the application values of real-time quantitative PCR-Sanger sequencing and TaqMan probe method in the detection of KRAS and BRAF mutations, and to correlate KRAS/BRAF mutations with the clinicopathological characteristics in colorectal carcinomas. METHODS: Genomic DNA of the tumor cells was extracted from formalin fixed paraffin embedded (FFPE) tissue samples of 344 colorectal carcinomas by...
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