Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Influence of the length of target DNA overhang proximal to the array surface on discrimination of single-base mismatches on a 25-mer oligonucleotide array.

BMC research notes - 17 Apr 2014

Tomlinson Jenny, Harrison Catherine, Boonham Neil, Goodchild Sarah A, Weller Simon A

Abstract excerpt

BACKGROUND: The performance of probes on an oligonucleotide microarray can be characterised in terms of hybridisation signal strength and the ability to discriminate sequence mismatches between the probe and the hybridising target strand, such as those resulting from SNPs. Various properties of the probe affect mismatch discrimination, such as probe length and the position of mismatched bases, and the effects of...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Base Pair Mismatch
Share to Base Pairing

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Base Sequence

Share to DNA Primers

Share to Nucleic Acid Hybridization

Share to Oligonucleotide Array Sequence Analysis

Share to Polymerase Chain Reaction

Source

Open in PubMed Open full text

Identifiers

PMID: 24742004
DOI: 10.1186/1756-0500-7-251
PMCID: PMC3997201

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 1 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Influence of the length of target DNA overhang proximal to the array surface on discrimination of single-base mismatches on a 25-mer oligonucleotide array.PMID 24742004Read ↗

Strong position-dependent effects of sequence mismatches on signal ratios measured using long oligonucleotide microarrays.Semantic proximity Read ↗

Rapid discrimination of single-nucleotide mismatches using a microfluidic device with monolayered beads.Semantic proximity Read ↗

Multi-stringency wash of partially hybridized 60-mer probes reveals that the stringency along the probe decreases with distance from the microarray surface.Semantic proximity Read ↗

Analysis of the factors affecting the accuracy of detection for single base alterations by oligonucleotide microarray.Semantic proximity Read ↗

Cantilever-based optical deflection assay for discrimination of DNA single-nucleotide mismatches.Semantic proximity Read ↗

Mismatch and G-stack modulated probe signals on SNP microarrays.Semantic proximity Read ↗

Guidelines for incorporating non-perfectly matched oligonucleotides into target-specific hybridization probes for a DNA microarray.Semantic proximity Read ↗

Free energy of DNA duplex formation on short oligonucleotide microarrays.Semantic proximity Read ↗

Effect of secondary structure on single nucleotide polymorphism detection with a porous microarray matrix; implications for probe selection.Semantic proximity Read ↗

Highly sensitive PNA array platform technology for single nucleotide mismatch discrimination.Semantic proximity Read ↗

Hybridization of DNA targets to glass-tethered oligonucleotide probes.Semantic proximity Read ↗

Detection of oligonucleotide systematic mismatches with a surface plasmon resonance sensor.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.