Article
Guidelines for incorporating non-perfectly matched oligonucleotides into target-specific hybridization probes for a DNA microarray.
Nucleic acids research - 1 Jan 2004
Lee Inhan, Dombkowski Alan A, Athey Brian D
Abstract excerpt
Sequence-specific oligonucleotide probes play a crucial role in hybridization techniques including PCR, DNA microarray and RNA interference. Once the entire genome becomes the search space for target genes/genomic sequences, however, cross-hybridization to non-target sequences becomes a problem. Large gene families with significant similarity among family members, such as the P450s, are particularly problematic....
Topics
- Base Sequence
- Carrier Proteins
- Cell Adhesion Molecules
- DNA Probes
- Humans
- Membrane Proteins
- Multigene Family
- Nucleic Acid Hybridization
- Oligodeoxyribonucleotides
- Oligonucleotide Array Sequence Analysis