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Article

Highly sensitive PNA array platform technology for single nucleotide mismatch discrimination.

Journal of microbiology and biotechnology - 1 Feb 2010

Choi Jae-Jin, Jang Minjeong, Kim Jihyun, Park Heekyung

Abstract excerpt

Reliable discrimination of single nucleotide mismatch was demonstrated using arrays with peptide nucleic acid (PNA) probes. Newly developed PNA probes immobilization method and hybridization conditions for PNA arrays gave excellent specificity and sensitivity. And we compared the specificity, sensitivity, and stability obtained with the PNA and DNA arrays in discriminating single nucleotide mismatches. PNA arrays...

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PMID: 20208431

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