Article
Hybridization of DNA targets to glass-tethered oligonucleotide probes.
Molecular biotechnology - 1 Dec 1995
Beattie W G, Meng L, Turner S L, Varma R S, Dao D D, Beattie K L
Abstract excerpt
Hybridization of nucleic acids to surface-tethered oligonucleotide probes has numerous potential applications in genome mapping and DNA sequence analysis. In this article, we describe a simple standard protocol for routine preparation of terminal amine-derivatized 9-mer oligonucleotide arrays on ordinary microscope slides and hybridization conditions with DNA target strands of up to several hundred bases in...
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