Article
Cantilever-based optical deflection assay for discrimination of DNA single-nucleotide mismatches.
Analytical chemistry - 1 Apr 2001
Hansen K M, Ji H F, Wu G, Datar R, Cote R, Majumdar A, Thundat T
Abstract excerpt
Characterization of single-nucleotide polymorphisms is a major focus of current genomics research. We demonstrate the discrimination of DNA mismatches using an elegantly simple microcantilever-based optical deflection assay, without the need for external labeling. Gold-coated silicon AFM cantilevers were functionalized with thiolated 20- or 25-mer probe DNA oligonucleotides and exposed to target oligonucleotides...
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