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Article

Optimization of a Lambda-RED Recombination Method for Rapid Gene Deletion in Human Cytomegalovirus.

International journal of molecular sciences - 29 Sept 2021

García-Ríos Estéfani, Gata-de-Benito Julia, López-Siles Mireia, McConnell Michael J, Pérez-Romero Pilar

Abstract excerpt

Human cytomegalovirus (HCMV) continues to be a major cause of morbidity in transplant patients and newborns. However, the functions of many of the more than 282 genes encoded in the HCMV genome remain unknown. The development of bacterial artificial chromosome (BAC) technology contributes to the genetic manipulation of several organisms including HCMV. The maintenance of the HCMV BAC in E. coli cells permits the...

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Share to Bacteriophage lambda
Share to Chromosomes, Artificial, Bacterial

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Cytomegalovirus

Share to Cytomegalovirus Infections

Share to Genome, Viral

Share to HEK293 Cells

Share to Recombination, Genetic

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Source

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Identifiers

PMID: 34638896
DOI: 10.3390/ijms221910558
PMCID: PMC8508972

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