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Article

Genetic method to analyze essential genes of Escherichia coli.

Applied and environmental microbiology - 1 Nov 2007

Hupert-Kocurek Katarzyna, Sage Jay M, Makowska-Grzyska Magdalena, Kaguni Jon M

Abstract excerpt

The genetic analysis of essential genes has been generally restricted to the use of conditional mutations, or inactivating chromosomal mutations, which require a complementing plasmid that must either be counterselected or lost to measure a phenotype. These approaches are limited because they do not permit the analysis of mutations suspected to affect a specific function of a protein, nor do they take advantage...

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Identifiers

PMID: 17873068
DOI: 10.1128/AEM.01756-07
PMCID: PMC2074942

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Source receipt: pubmed, retrieved 7 Sept 2026.

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