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Targeted gene knockout and essentiality testing by homologous recombination.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2015

Gopinath Krishnamoorthy, Warner Digby F, Mizrahi Valerie

Abstract excerpt

This chapter provides an updated experimental protocol for generating allelic exchange mutants of mycobacteria by two-step selection using the p2NIL/pGOAL system. The types of mutants that can be generated using this approach are targeted gene knockouts marked with a drug resistance gene, unmarked deletion mutants, or strains in which a point mutation/s has been introduced into the target gene. A method for...

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PMID: 25779314
DOI: 10.1007/978-1-4939-2450-9_8

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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