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Article

Improvement of stability and enzymatic activity by site-directed mutagenesis of E. coli asparaginase II.

Biochimica et biophysica acta - 1 Jul 2014

Verma Shikha, Mehta Ranjit Kumar, Maiti Prasanta, Röhm Klaus-Heinrich, Sonawane Avinash

Abstract excerpt

Bacterial asparaginases (EC 3.5.1.1) have attracted considerable attention because enzymes of this group are used in the therapy of certain forms of leukemia. Class II asparaginase from Escherichia coli (EcA), a homotetramer with a mass of 138 kDa, is especially effective in cancer therapy. However, the therapeutic potential of EcA is impaired by the limited stability of the enzyme in vivo and by the induction of...

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Source

Identifiers

PMID: 24721562
DOI: 10.1016/j.bbapap.2014.03.013

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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