Article
Structural and biophysical studies of new L-asparaginase variants: lessons from random mutagenesis of the prototypic Escherichia coli Ntn-amidohydrolase.
Acta crystallographica. Section D, Structural biology - 1 Jul 2022
Loch Joanna I, Klonecka Agnieszka, Kądziołka Kinga, Bonarek Piotr, Barciszewski Jakub, Imiolczyk Barbara, Brzezinski Krzysztof, Gilski Mirosław, Jaskolski Mariusz
Abstract excerpt
This work reports the results of random mutagenesis of the Escherichia coli class 2 L-asparaginase EcAIII belonging to the Ntn-hydrolase family. New variants of EcAIII were studied using structural, biophysical and bioinformatic methods. Activity tests revealed that the L-asparaginase activity is abolished in all analyzed mutants with the absence of Arg207, but some of them retained the ability to undergo the...
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