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Article

Purification of Escherichia coli L-asparaginase mutants by a native polyacrylamide gel electrophoresis.

Journal of chromatographic science - 1 Jul 2008

Wei Yujun, Chen Jianhua, Jia Ruibo, Wang Min, Wu Wutong

Abstract excerpt

The antigenicity of L-asaparaginase (L-ASP) has been problematic for the treatment of leukemia for many years. In order to establish a relationship between the antigenic epitope of L-asparaginase and its antigenicity, several L-asparaginase mutants (mL-ASPs) are constructed and expressed. To effectively purify these enzyme mutants for further investigation, a native preparative polyacrylamide gel electrophoresis...

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Identifiers

PMID: 18647480
DOI: 10.1093/chromsci/46.6.556

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 5 Sept 2026. Manifest publication-graph-cohort-2026-09-05.1.

Source receipt: pubmed, retrieved 5 Sept 2026.

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