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Efficient genetic editing of human intestinal organoids using ribonucleoprotein-based CRISPR

2023-03-31

Abstract excerpt

Organoids are currently one of the most widely used ex vivo models in epithelial biology. Combined with genetic editing strategies, organoids offer a promise of rapid and efficient investigation of gene function in many models of human disease. However, to date, the editing efficiency of organoids with the use of non-viral electroporation methods has been only up to 30%, with implications for the subsequent need...

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Literature Corpus work
9e6e0d5b-2ab1-59fa-bd38-74d914e861d1
DOI
10.1101/2023.03.31.535108
Open publication

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Efficient genetic editing of human intestinal organoids using ribonucleoprotein-based CRISPRDOI 10.1101/2023.03.31.535108
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