Article

Human Dicer1 hotspot mutation induces both loss and gain of miRNA function

2025-10-15

Abstract excerpt

The core miRNA biogenesis enzyme Dicer1 sustains recurrent mutations in cancer that compromise its RNase IIIb domain, which cleaves 5p arms of pre-miRNA hairpins. However, the lack of knockin models has limited fuller understanding. Here, we generated Dicer1-KO and Dicer1-S1344L (homozygous and hemizygous) human ESCs; the latter is a non-catalytic mutation in RNase IIIa that impairs RNase IIIb activity. Dicer1...

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Literature Corpus work: 1d16343b-c7db-5f2f-94f2-8ea48e8b5f25
DOI: 10.1101/2025.10.15.682667

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