MAPREC assay for quantitation of mutants in a recombinant flavivirus vaccine strain using near-infrared fluorescent dyes.

doi:10.1016/j.jviromet.2011.04.008

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Article

MAPREC assay for quantitation of mutants in a recombinant flavivirus vaccine strain using near-infrared fluorescent dyes.

Journal of virological methods - 1 Jul 2011

Bidzhieva Bella, Laassri Majid, Chumakov Konstantin

Abstract excerpt

Mutant analysis by PCR and restriction enzyme cleavage (MAPREC) is a quantitative assay of revertants in batches of live viral vaccines. The assay is highly sensitive and reliable but requires radioactive isotopes, which complicates its use in quality control laboratories. To quantify mutants in the cDNA of the West Nile (WN)/Dengue 4 chimera that was proposed as a new candidate of live vaccine against West Nile...

Topics

DNA Restriction Enzymes
Dengue Virus
Flavivirus
Fluorescent Dyes
Mutation
Poliovirus
Polymerase Chain Reaction
Restriction Mapping
Vaccines, Synthetic
Viral Vaccines
West Nile virus

Source

Open in PubMed

Identifiers

PMID: 21514323
DOI: 10.1016/j.jviromet.2011.04.008

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