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Article

The Use of Next-Generation Sequencing for the Quality Control of Live-Attenuated Polio Vaccines.

The Journal of infectious diseases - 9 Nov 2020

Charlton Bethany, Hockley Jason, Laassri Majid, Wilton Thomas, Crawt Laura, Preston Mark, Rigsby Peter, Chumakov Konstantin, Martin Javier

Abstract excerpt

BACKGROUND: Next-generation sequencing (NGS) analysis was compared to the current MAPREC (mutational analysis by polymerase chain reaction and restriction enzyme cleavage) assay for quality control of live-attenuated oral polio vaccine (OPV). METHODS: MAPREC measures reversion of the main OPV attenuating mutations such as uracil (U) to cytosine (C) at nucleotide 472 in the 5' noncoding region of type 3 OPV....

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Source

Identifiers

PMID: 32492703
DOI: 10.1093/infdis/jiaa299

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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