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Article

Efficient genome modification by CRISPR-Cas9 nickase with minimal off-target effects.

Nature methods - 1 Apr 2014

Shen Bin, Zhang Wensheng, Zhang Jun, Zhou Jiankui, Wang Jianying, Chen Li, Wang Lu, Hodgkins Alex, Iyer Vivek, Huang Xingxu, Skarnes William C

Abstract excerpt

Bacterial RNA-directed Cas9 endonuclease is a versatile tool for site-specific genome modification in eukaryotes. Co-microinjection of mouse embryos with Cas9 mRNA and single guide RNAs induces on-target and off-target mutations that are transmissible to offspring. However, Cas9 nickase can be used to efficiently mutate genes without detectable damage at known off-target sites. This method is applicable for...

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Share to CRISPR-Cas Systems
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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Source

Identifiers

PMID: 24584192
DOI: 10.1038/nmeth.2857

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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