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Article

Conditional gene silencing of multiple genes with antisense RNAs and generation of a mutator strain of Escherichia coli.

Nucleic acids research - 1 Aug 2009

Nakashima Nobutaka, Tamura Tomohiro

Abstract excerpt

In this study, we describe a method of simultaneous conditional gene silencing of up to four genes in Escherichia coli by using antisense RNAs. We used antisense RNAs with paired termini, which carried flanking inverted repeats to create paired double-stranded RNA termini; these RNAs have been proven to have high silencing efficacy. To express antisense RNAs, we constructed four IPTG-inducible vectors carrying...

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Identifiers

PMID: 19515932
DOI: 10.1093/nar/gkp498
PMCID: PMC2731896

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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