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Article

Ultra-deep pyrosequencing (UDPS) data treatment to study amplicon HCV minor variants.

PloS one - 1 Jan 2013

Gregori Josep, Esteban Juan I, Cubero María, Garcia-Cehic Damir, Perales Celia, Casillas Rosario, Alvarez-Tejado Miguel, Rodríguez-Frías Francisco, Guardia Jaume, Domingo Esteban, Quer Josep

Abstract excerpt

We have investigated the reliability and reproducibility of HCV viral quasispecies quantification by ultra-deep pyrosequencing (UDPS) methods. Our study has been divided in two parts. First of all, by UDPS sequencing of clone mixes samples we have established the global noise level of UDPS and fine tuned a data treatment workflow previously optimized for HBV sequence analysis. Secondly, we have studied the...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Share to High-Throughput Nucleotide Sequencing

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Share to RNA, Viral

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Source

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Identifiers

PMID: 24391758
DOI: 10.1371/journal.pone.0083361
PMCID: PMC3877031

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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