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Minor variant detection in amplicons using 454 massive parallel pyrosequencing: experiences and considerations for successful applications.

BioTechniques - 1 Sept 2011

Vandenbroucke Ina, Van Marck Herwig, Verhasselt Peter, Thys Kim, Mostmans Wendy, Dumont Stéphanie, Van Eygen Veerle, Coen Katrien, Tuefferd Marianne, Aerssens Jeroen

Abstract excerpt

Ultra-deep sequencing (UDS) of amplicons is a major application for next-generation sequencing technologies, even more so for the 454 Genome Sequencer FLX. Especially for this application, errors that might be introduced during any of the sample processing or data analysis steps should be avoided...

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PMID: 21906038
DOI: 10.2144/000113733

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 4 Sept 2026. Prepared record publication-21906038-2026-09-04.2. Manifest publication-graph-cohort-2026-09-04.2.

Source receipt: PubMed via Helena Literature Corpus, retrieved 4 Sept 2026.

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