Article
Application of the ASLP technology to a novel platform for rapid and noise-free multiplexed SNP genotyping.
Biosensors & bioelectronics - 15 Apr 2014
Shin Sung Chul, Kim Gahee, Yang Hee-Bum, Park Kwan Woo, Kang Byoung-Cheorl, Park Hyun Gyu
Abstract excerpt
A novel multiplexing method, which relies on universal amplification of separated ligation-dependent probes (ASLP), has been developed to genotype single-nucleotide polymorphisms (SNPs). The ASLP technique employs two allele-specific oligonucleotides (ASO), modified with universal forward primer sequences at the 5'-end and a common locus-specific oligonucleotide (LSO) extended with a universal separation (US)...
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