Article
An integrated allele-specific polymerase chain reaction-microarray chip for multiplex single nucleotide polymorphism typing.
Lab on a chip - 21 Dec 2012
Choi Jong Young, Kim Yong Tae, Byun Ju-Young, Ahn Jinwoo, Chung Soyi, Gweon Dae-Gab, Kim Min-Gon, Seo Tae Seok
Abstract excerpt
An integrated allele-specific polymerase chain reaction (AS PCR) and microarray chip has been developed for multiplex single nucleotide polymorphism (SNP) typing on a portable genetic analyzer instrumentation. We applied the integrated PCR-microarray system for on-site Hanwoo (Korean indigenous beef cattle) identification. Eleven sets of primers were designed, among which ten sets of primers targeted ten SNP loci...
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