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Application of partially double-stranded DNA probes to high-throughput SNPs genotyping.

Nucleic acids symposium series (2004) - 1 Jan 2008

Ishii Tomoya, Muraki Kentaro, Shimada Naohiko, Kano Arihiro, Nishida Nao, Tokunaga Katsushi, Maruyama Atsushi

Abstract excerpt

We have focused on DNA strand exchange reaction (SER) with partially double-stranded (PDS) probes as a novel platform of SNPs genotyping. In this report, we elaborated the method for reliable and high-throughput SNPs typing. Competitive reactions using a couple of PDS probes designed for wild and mutant sequences significantly increase resolution efficiency to SNP types that is hardly discriminated by a...

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PMID: 18776341
DOI: 10.1093/nass/nrn120

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