Article
Fabrication and application of single nucleotide polymorphisms library on magnetic nanoparticles using adaptor PCR.
Journal of nanoscience and nanotechnology - 1 Jan 2008
Liu Hongna, Li Song, Ji Meiju, Nie Libo, Chen Jianrong, Miao Yuqing, He Nongyue
Abstract excerpt
We have developed a novel approach to fabricate single nucleotide polymorphisms (SNPs) library on magnetic nanoparticles (MNPs) based on adaptor PCR. Each SNP locus in the library was interrogated by hybridization with a pair of allele specific dual-color fluorescence (Cy3, Cy5) probes to determine SNP. Two SNPs loci (M235T and A-6G) associated with essential hypertension in the angiotensinogen (AGT) gene were...
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