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Article

Optimization of PCR conditions for amplification of GC-Rich EGFR promoter sequence.

Journal of clinical laboratory analysis - 1 Nov 2013

Obradovic Jasmina, Jurisic Vladimir, Tosic Natasa, Mrdjanovic Jasminka, Perin Branislav, Pavlovic Sonja, Djordjevic Natasa

Abstract excerpt

BACKGROUND: Polymerase chain reaction (PCR) is an extremely sensitive method that often demands optimization, especially when difficult templates need to be amplified. The aim of the present study was to optimize the PCR conditions for amplification of the epidermal growth factor receptor (EGFR) promoter sequence featuring an extremely high guanine-cytosine (GC) content in order to detect single nucleotide...

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Source

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Identifiers

PMID: 24218132
DOI: 10.1002/jcla.21632
PMCID: PMC6807403

Genes

EGFR

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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