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Article

Genetic Manipulation of the Plant Pathogen Ustilago maydis to Study Fungal Biology and Plant Microbe Interactions.

Journal of visualized experiments : JoVE - 30 Sept 2016

Bösch Kristin, Frantzeskakis Lamprinos, Vraneš Miroslav, Kämper Jörg, Schipper Kerstin, Göhre Vera

Abstract excerpt

Gene deletion plays an important role in the analysis of gene function. One of the most efficient methods to disrupt genes in a targeted manner is the replacement of the entire gene with a selectable marker via homologous recombination. During homologous recombination, exchange of DNA takes place between sequences with high similarity. Therefore, linear genomic sequences flanking a target gene can be used to...

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Identifiers

PMID: 27768088
DOI: 10.3791/54522
PMCID: PMC5092128

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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