Article
Transformation-associated recombination (TAR) cloning for genomics studies and synthetic biology.
Chromosoma - 1 Sept 2016
Kouprina Natalay, Larionov Vladimir
Abstract excerpt
Transformation-associated recombination (TAR) cloning represents a unique tool for isolation and manipulation of large DNA molecules. The technique exploits a high level of homologous recombination in the yeast Sacharomyces cerevisiae. So far, TAR cloning is the only method available to selectively recover chromosomal segments up to 300 kb in length from complex and simple genomes. In addition, TAR cloning allows...
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