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Efficient multiple gene knock-out in <i>Colletotrichum higginsianum</i> via CRISPR-Cas9 ribonucleoprotein and <i>URA3</i> -based marker recycling

2023-04-20

Abstract excerpt

Colletotrichum higginsianum is a hemibiotrophic pathogen that causes anthracnose disease on crucifer hosts, including Arabidopsis thaliana. Despite the availability of genomic and transcriptomic information and the ability to transform both organisms, identifying C. higginsianum genes involved in virulence has been challenging due to their recalcitrance to gene targeting and redundancy of virulence factors. To ove...

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Literature Corpus work
ab2926ae-2aa6-5595-98cc-db7000d71c5b
DOI
10.1101/2023.04.20.537420
Open publication

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Efficient multiple gene knock-out in <i>Colletotrichum higginsianum</i> via CRISPR-Cas9 ribonucleoprotein and <i>URA3</i> -based marker recyclingDOI 10.1101/2023.04.20.537420
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